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Diyala Journal For Pure Science

Scientific Refereed Journal Published By College of Science - University of Diyala


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Diyala Journal For Pure Sciences DJPS

ISRA Impact Factor:3.715

P- ISSN:2222-8373, E-ISSN:2518-9255

Volume 13, Issue 2, Part 1 , April. 2017


Assessment of bacterial contamination and antibiotics susceptibility of pathogenic Bacteria in the operating theaters in AL-Batool teaching hospital


Burooj Mohammed Razooqi


Year: 2017, Volume: 13, Issue: 2, Part: 1


Pages: 174-184, DOI: http://dx.doi.org/10.24237/djps.1302.195A


Language: English


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Back ground: - Surgical wound pathogens is one of the most complica­tions in every kinds of surgery. Surgical pathogens can be fatal or lead to a poorly diagnosis, causing critical trouble to both patients and doctors.

Objective:-To assess of the contamination rate and antibiotics susceptibility of pathogenic bacteria in the operating theaters in Al-Batool teaching hospital.

Materials and Methods :- This study was done in the Al-Batool  teaching hospital in Diyala province for the period from one July   2015 to one February 2016 .192 swabs were taken from different sites from operating theaters included:-operative couch ,wall and floors ,air outlets ,surgical instrument ,water tape, door knob, guess . Were taken cultured on different culture media and submitted to a serial of different biochemical tests for the diagnosis of type of bacteria. and the cultures were tested for antibiotics sensitive to evaluate their activity  against pathogenic bacteria isolated from operating theaters.

Results :- In  this study taken 192 swabs , 160 (83.33 %) swabs  show positive results for bacterial growth as single and mixed isolate, and 32(16.66 %)were negative (no growth). from 160 positive swabs were  202 single positive bacterial isolates, 116(57.42 %) gram negative bacteria, which include 66(56.89 %) Escherichia coli , 21( 18.1%)Enterobacter sp.,12(10.34%) klebsiella sp.,and11(9.48%) for pseudomonas aeruginosa . while 86( 42.57%) of bacterial isolates were gram positive include 49 (56.97 %) Staphylococcus epidermidis and 15 (17.44%) Staphylococcus aureus . The  result shows high percentage of contamination with gram negative bacteria 38 (32.75 %) and26(22.41 %) during September and October while shows high percentage of contamination with gram positive bacteria 24(27.9%)and17,(19.76%)during January and December .The Cephalothin ,Cefotaxime and Rifampin are more effective against study isolates.

Conclusion:- Contamination of operating theaters was the most dangerous problem .Which was  responsible for 90% of opportunistic infections in hospital ,which may be came   from another  person , from patients flora ,or by contamination instruments in the operating rooms ,the rate of bacterial contamination was high during September and October. 


Keywords:-Escherichie.coli ,Staphylococcus aureus , Operative theaters, surgical instruments .National nosocomial infection ,Antibiotics sensitivity.

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