Diyala Journal For Pure Science
Scientific Refereed Journal Published By College of Science - University of Diyala
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Diyala Journal For Pure Sciences DJPS
ISRA Impact Factor:3.715
P- ISSN:2222-8373, E-ISSN:2518-9255
Volume 13, Issue 2, Part 1 , April. 2017
Identification Of Staphylococcus Aureus Isolated From Different Infections And Study The Ability Of Nuclease Production
Ansam Dawod Salman and Eman Abass Ali
Year: 2017, Volume: 13, Issue: 2, Part: 1
Pages: 215-225, DOI: http://dx.doi.org/10.24237/djps.1302.208B
This study was conducted for the period from 1/3/2016 to 15/6/2016 in Baquba city in Iraq. Eighty samples were collected from different infections from Baquba General Hospital and AL-Batool Hospital. Twenty isolates (25%) were found to be Staphylococcus aureus . The susceptibility test was applied on these isolates against (12) antibiotics. The results revealed that the highest resistances were for Penicillin and Methicillin with 100% for each, while the lowest resistance were for Erythromycin (6.3%) and Vancomycin (9.1%). The results of virulence factors that had S. aureus showed possession of all isolates many virulence factors and a high production of which increases the pathoginicity of it. All isolates were able to produce urease (100%), heamolycin (100%), protease (85%), lipase (80%) and gelatinase (100%). Twenty isolates were screening for nuclease production, the isolates SA9 and SA19 were found to be an efficient nuclease production, 30mm the diameter of clear zone around colonies were cultured on DNase agar.
Key wards: Staphylococcus aureus, nuclease production, Antibiotics.