Diyala Journal For Pure Science
Scientific Refereed Journal Published By College of Science - University of Diyala
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Diyala Journal For Pure Sciences DJPS
ISRA Impact Factor:3.715
P- ISSN:2222-8373, E-ISSN:2518-9255
Volume 13, Issue 1, Part 1 , January. 2017
Heavy Metals Tolerance and Antibiotics Susceptibilty Profiles of Staphylococcus aureus Strains Isolated From Clinical Sources in Baquba city
Lina Abdulameer Salman Alsaadi
Year: 2017, Volume: 13, Issue: 1, Part:1
Pages: 130-144, DOI: http://dx.doi.org/10.24237/djps.1301.136A
Twenty- one Staphylococcus aureus isolated from various clinical samples (wound , ear , pus , urin and burn ) collected from Baquba General Hospital over a period ( April 2015 to June 2015 ).Out of 21 Staphylococcus aureus isolates , 17 (80.95%) were resistant to methicillin , while 4 (19.04%) were methicillin susceptible. Results of antimicrobial susceptibility showed high resistance to amoxicillin , amoxicillin/clavulanic acid , trimethoprime and cefotaxime with ratio 100% , 76.1% , 76.1% and 71.4% respectively, while isolates resistant oflaxacin with 4.7%. The results showed that 15 isolates of the total (21) of Staphylococcus aureus could produce β-lactamase with percentage (71.4%) , 5 isolates produce of Metalloβ-lactamase with (23.80% ) , and 4 isolates (19.04%) produce ESβLs. Staphylococcus aureus isolates have ability to tolerant the highest concentration of heavy metals like (Cobalt , Copper , iron , Mercury , Zinc) with ratio (57.1% , 52.3% , 66.6% , 33.3% , 61.9%) respectively. Additionally result of plasmid profile presented that all isolates of Staphylococcus aureus contained two band s of plasmid vary in size . Plasmid curing was carried out by acridin orang (256µg/ml) , cobalt and zinc resistance character was found to be present on the chromosomal DNA rather than the plasmid DNA whereas iron , copper and mercury resistance characters were found to be present on the plasmid . Curing result showed the loss of antibiotic and heavy metal resistance property for (cefotaxim , amoxicillin , erythromycin , ciprofloxacin , iron , copper and mercury ) from the isolates and confirms a relationship between antibiotic and heavy metal resistance with plasmid.
Key words : Staphylococcus aureus , Heavy Metals , Antibiotics , Curing, Plasmid .