Diyala Journal For Pure Science
Scientific Refereed Journal Published By College of Science - University of Diyala
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Diyala Journal For Pure Sciences DJPS
ISRA Impact Factor:3.715
P- ISSN:2222-8373, E-ISSN:2518-9255
Volume 14, Issue 1, Part 1 , Januar y. 2018
Single Nucleotide Polymorphism of Interleukin-6 Gene at Position +565 in Type-1 Diabetic Patients in Baqubah City, Diyala, Iraq
Maha Falh Nazzal
Year: 2018, Volume: 14, Issue: 1, Part:1
Pages: 183-190 , DOI: http://dx.doi.org/10.24237/djps.1401.369A
This study aimed to investigate the potential association between the polymorphism of the Interlukin-6 gene (G˃C) at position + 565 SNP. and type 1 diabetes (T1D) in Iraqi patients, the study included 39 blood samples which have withdrawn from diabetic patients (12 males and 27 females and an average age of 15.65 ± 1.79 years) who were diagnosed by a medical staff at Baquba General Hospital for the period from October 2015 to March 2016 together with 21 blood samples from apparently healthy people as a control group (7 males and females and an average age of 14.26 ±1.43 years). The polymerase chain reaction-specific sequence primer (PCR-SSP) assay has been used in this study. The results revealed that comparing IL6+590 genotypes and alleles between T1D patients and controls showed some significant variance. The results of the gel electrophoresis of the IL-6 G / C + 565 gene amplified by PCR-SSP showed that the two genes G and C were present and that there were three genotypes: GG, GC, CC among patients. The results showed that frequency of GG genotype (56.41 vs 28.57%; respectively) and G allele (74.36 vs 59.52%; RR =36.7%) was significantly increased in diabetic patients in comparison with the controls (P = 0.058 and 0.102, respectively) and the related RR rates were 39.0% and 36.7%, respectively, while the associated EF values were 3.24 and 1.97, respectively. In contrast, GC genotype (35.89 vs 61.90%; P =0.063; PF =0.34) and C allele (25.64 vs 40.48%; P =0.102; PF =0.51) frequencies were significantly decreased in patients. The findings of this study depict the predominant pathogenic role of IL-6+565 polymorphism showed associations (positive and negative) with T1D in the samples of Iraqi patients. Therefore, the functional role IL6 might have been altered due to the deviations of some genotype and allele frequencies pathogenesis of T1D. This leads to the role of IL-6 as a preventive agent against the disease because it acts as an anti-inflammatory and inflammatory generator at the same time.
Keywords: Type-1 Diabetes disease, IL-6 polymorphism, Autoimmune disease.