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DJPS

Diyala Journal For Pure Science

Scientific Refereed Journal Published By College of Science - University of Diyala

 

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djps.1403.425B

Diyala Journal For Pure Sciences DJPS

ISRA Impact Factor:3.715

P- ISSN:2222-8373, E-ISSN:2518-9255

Volume 14, Issue 3 , July . 2018

 

 

 

Effect of Lactobacillus Bacteria on the Growth of Escherichia coli Isolated from Infants with Amoebic Dysentery Infections

 

Kareem Ibrahim Mubaruk , Doaa Mahmood Mahady

 

Year: 2018, Volume: 14, Issue: 3

 

Pages: 123 -  136, DOI: http://dx.doi.org/10.24237/djps.1403.425B 

 

Language: English

 

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Abstract


 

This study was done in Al Batoul Teaching Hospital from 25/ 9/2016 to 1/2/2017 and 200 stool samples were collected from infants suffer from amoebic dysentery diarrhea with the age less than two years. All stool samples were examined for general stool analysis, cultured on MacConkey agar, the colony characterstic were thoroughly investigated, other biochemical tests were done according to standard procedures, then diagnosed by Api20E and VITEK 2. Fifty (40%) isolates of E. coli were recovered. The E. coli isolates showed different degrees of resistance to cephalosporines so that the resistant rate to Cefixime, Cefuroxime, Ceftriaxone and Cefoxitin were 88%, 90%, 84% and 12%, respectively. The sensitive ratio of all isolates to Meropenem antibiotic was 92%. The result showed that MIC values of the Cefixime ranged between 32 and 512 μg / ml, Cefuroxime, Ceftriaxone 32 and 1024 μg / mL and Tetracyclin 16 and 512 μg / ml. The current study showed that 96% of isolates from the infant's stool have the ability to produce hemolysin, whereas 4% of the isolates produce bacteriocin with a very high significant statistical difference (P = 0.001). Lactobacillus bacteria were isolated from the stools of very young children and were dependent on breastfeeding by 15%, and 11.4% were obtained from the vaginal wall swabs, the colony morphology was thoroughly investigated and other biochemical tests were done for identification. One isolate was selected as probiotic to determine the inhibitory effect against E. coli and the diameter mean of the inhibition zone on 50 isolates of E. coli was 22.82 mm. It was concluded that Lactobacillus bacteria are useful as probiotics for the treatment of diarrhea caused by E. coli.

 

Keywords: E. coli, antibiotic, diarrhea infant, Lactobacillus.

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